Simple, precise and accurate zero order derivative spectroscopic method has been developed and validated for the estimation of Quetiapine fumarate in bulk and pharmaceutical dosage form. The drug shows maximum absorption (λ max) at 226nm in Methanol: 1N NaOH (50:50) solution and obeys Beer’s law in the concentration range of 2- 10μg/ml. The linearity study was carried out and regression coefficient was found to be 0.9996=b and it has showed good linearity, precision during this concentration range. The % recovery was found to be 100.81-101.35. The LOD and LOQ were found to be 0.388 and 1.17μg/ml. The percentage relative standard deviation were found to be less than 2. Aa per the ICH guidelines the technique has been validated for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for routine quantification of Quetiapine fumarate in bulk and pharmaceutical dosage form.
Simple, precise and accurate zero order derivative spectroscopic method has been developed and validated for the estimation of Bedaquiline (sirturo 100mg) in bulk and pharmaceutical dosage form. The drug shows maximum absorption (λ max) at 226nm in Methanol solution and obeys Beer’s law in the concentration range of 0.5-2.5µg/ml. The linearity study was carried out and regression coefficient was found to be 0.9999 and it has showed good linearity, precision during this concentration range. The % recovery was found to be 96.88-99.59. The LOD and LOQ were found to be 0.0254 and 0.0770µg/ml. The percentage relative standard deviation is found to be less than 2. As per ICH guidelines the technique has been validated for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for routine quantification of Bedaquiline in bulk and pharmaceutical dosage form.
S. Chethan Gowda*, C. Jose Gnana Babu, H. G. Sowmya.
A Rapid and simple procedure free from exiting protocol with avoid of toxic, costly catalyst. This catalyst free green synthetic reaction as a retrievable and utility green solvent in reaction fashion clearly displayed a high attraction in the vicinity of green approach is efficient step to improved atomic economic for the synthesis of pyrazoles derivatives in microwave method via multi-component one pot synthesis from 1, 3 diketones with hydrazine hydrates in glycerol solvent media this modern strategy could serve as a valuable synthetic alternative for green synthesis reactions process diverge in terms of economic aspects and pharmaceutical profiles of novel pyrazoles derivatives. Chemical Structures confirmed by FT-IR, Proton and Carbon NMR spectral data. We investigate In vitro antibacterial activity of synthesized compounds against, St.aureus, Streptococci, E-coli etc.
The synthesis of dihydro pyrimidines were done using chalcone Derivatives with glacial acetic caid and sodium acetate to form the cyclic heterocyclic structure. The formed compounds were characterised by using IR, NMRspectroscopy. The anti-microbial activity was performed using diffusion method on the Gram positive and negative microorganisms. The antioxidant activity was performed using the DPPH method. The compound PR-06 shows better activity than other compounds due to presence of the electron withdrawing groups.
Two new synthesized and characterized quinazoline Mannich bases 1 and 2 were investigated for anticancer activity against MCF-7 human breast cancer cell line. Compounds 1 and 2 demonstrated a remarkable anti-proliferative effect, with an IC50 value of 6.035 × 10−6mol/L and 5.620 × 10−6mol/L, respectively, after 72 hours of treatment. Most apoptosis morphological features in treated MCF-7 cells were observed by AO/PI staining. The results of cell cycle analysis indicate that compounds did not induce S and M phase arrest in cell after 24 hours of treatment. Furthermore, MCF-7 cells treated with 1 and 2 subjected to apoptosis death, as exhibited by perturbation of mitochondrial membrane potential and cytochrome c release as well as increase in ROS formation. We also found activation of caspases-3/7, -8 and -9 in compounds 1 and 2. Our results showed significant activity towards MCF-7 cells via either intrinsic or extrinsic mitochondrial pathway and are potential candidate for further in vivo and clinical breast cancer studies.
