Chalcones are prepared by claisen Schmidt condensation method they are used to prepare various heterocyclic compounds. Most of them are widely used in pharmaceuticals. Keeping this in mind new chalcones are synthesised and the structures were confirmed by IR, NMR and elemental analysis. Synthesised compounds were screened for their antibacterial activity the molecules were screened for their structural activity relationships by atom based 3D QSAR studies.

The main objective of this study was to develop a simple, precise, specific and accurate RP-HPLC method for the simultaneous estimation of Metformin Hydrochloride (MET) and Gliclazide (GLZ) in bulk and combined dosage form. The separation method was carried out using Reverse phase C18 column; ZODIAC - 3V (250 mm x 4.6 mm x 5μm). Mixture of Phosphate buffer (1.625 gm of Potassium Di Hydrogen Ortho Phosphate and 0.3 gm of Di Potassium Hydrogen Ortho Phosphate in 550 ml water) using as mobile phase; pH 4.8 and Acetonitrile (55:45 (v/v)) at isocratic mode and eluents were monitored at 248 nm using UV- Visible spectrophotometer as the Detector with the optimized method, the Retention times of MET and GLZ were found to be 2.420 and 4.270 mnts respectively with theoretical plate count and asymmetry as per the ICH limits. The linearity range was good in this method and it was of 60-140μg/ml for Metformin and 3- 8μg/ml for Gliclazide with Regression coefficient (r2) of 0.999 and 0.999. The percentage assays were found to be 99.59% for MET and 98.13% for GLZ. Limit of detection and Limit of quantitation values were found to be 1.53μg/ml and 4.65μg/ml for Metformin Hydrochloride, 0.02μg/ml and 0.07μg/ml for Gliclazide respectively. The method was found to be Accurate (with percentage mean recoveries 101.14% for Metformin HCl and 100.22% for Gliclazide), precise, robust, stable and Specific. The proposed method was validated by using ICH guidelines and hence can be successfully applied to the simultaneous estimation of MET and GLZ in tablet formulations.

A simple, Accurate, precise method was developed for the simultaneous estimation of Ceftolozane and Tazobactum in Pharmaceutical dosage form. Chromatogram was run through Std ODS 250mm x 4.6 mm, 5m. Mobile phase containing Buffer (Ortho Phosphoric Acid), Acetonitrile in the ratio of (60:40) was pumped through column at a flow rate of 1ml/min. Temperature was maintained at 30°C. Optimized wavelength for Ceftolozane and Tazobactum was 210nm. Retention times of Ceftolozane and Tazobactum were found to be 2.335min and 3.850min. % RSD of the Ceftolozane and Tazobactum were and found to be 0.9 and 0.5 respectively. % Recover was Obtained as 99.30% and 99.67% for Ceftolozane and Tazobactum respectively. LOD, LOQ values obtained from regression equations of Ceftolozane and Tazobactum were 0.02, 0.03 and 0.07, 0.08, respectively. Regression equation of Ceftolozane is y =16395.x + 11260 and Tazobactum is y = 18140x + 5689. Regression co-efficient was 0.999. The method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Allium cepa is used as food additive and as medicine, due to their content of phytonutrients and used for the treatment as well as prevention of a number of diseases such as coronary heart disease, cancer, obesity, diabetes, hyper-cholesterolemia, disturbances of the gastrointestinal tract and inflammatory diseases. This review article focuses the phytochemical constituents and pharmacological activities of Allium cepa.

This study presents rapid, sensitive and accurate HPLC method for determinations of gliclazide in Rabbit Plasma (RP). Rabbit plasma samples were subjected to protein precipitation by methanol and protein free plasma samples were injected to HPLC C 18 coloums. Glipizide was used as internal standard. Acetonitrile and pH 6.8 Phosphate buffer at 50:50 ratio, at a flow rate of 1mL /min and a pressure of 150-200 Kg/cm 2 were used to get the chromatogram. A good separation of gliclazide and glipizide was achieved by this method with retentiontimes of 7.4 and 4.5 min respectively. The calibration curveof the gliclazide in a concentration range of 50-800 ng/mL resulted in the linear least square regression equation i.e. Y=0.032X+0.5187. Peak areas were reproducible as indicated by low coefficient of variation (<1.79%).

Piperine is used in many different ways to influence our lives. The piperine, in the spicy food, stimulates perspiration, which causes a cooling effect of the body. Thus very helpful during hot summer. Piperine (1-peperoyl piperidine), an amide alkaloid, is foundin various Pipperspecies and posses antioxidant, antiplatelet, anti-inflammatory, antihypertensive, hepatoprotective, antithyroid, antitumor, antiasthmatic activities. It also believed to be a fertility enhancer. Piperine act as absorption enhancer of many drugs and nutrients from gastrointestinal tract by various mechanisms and a potent inhibitor of drug metabolism by inhibiting various metabolizing enzymes. This review article gives an account of bioavailability enhancing property of piperine.

A simple, specific, precise, accurate, rapid and reproducible efficient reversed phase HPLC method with PDA detector has been developed and validation for simultaneous estimation of Lamivudine (LAM) and Raltegravir (RAL) in pharmaceutical dosage form. Chromatography was performed on aInertsil ODSC18column (150mmX4.6mm, 5.0μmparticlesize) with a 50:50v/v mixture of 0.1% orthophospharic acid buffer: acetonitrileasa mobile phase. The detection of the combined dosage form was carried out at 242nm and flow rate employed was0.9 ml/min. The retention times were 1.9±0.3 and 4.3±0.3min for Lamivudine and Raltegravil respectively. Linear was established in the concentration range of 15.0to75.0μg/ml for LAM and 30to150μg/ml for RAL with a correlation coefficient of bothdrugs for found to be 0.998 and 0.999. The recoveries obtained were 99.18-100.60% for LAM and 98.94-101.07% for RAL. Similarly the %RSD value for precision was also found to be within the acceptable limit. The method was validated according to international conference of harmonization guidelines in terms of accuracy, precision, specificity, robustness, linearity and other aspects of analytical validation. The results of the analysis were validated statistically and recovery studies confirmed the accuracy and precision of the proposed method. Developed method was rapid and convenient which could be successfully applied for the routine control of both the component.

Since resistance of pathogens towards currently available drug therapies is rapidly becoming a major problem, the design of new compounds to deal with fungus resistance has become one of the most important areas of antibacterial research today. Keeping in view of the above, here in, we have described the synthesis of a series of novel 3-(substituted phenyl)-N-(4H-1, 2, 4-triazol-4-yl) acrylamide derivatives (3a, 3b and 3c) as anti-fungal agents. These compounds were prepared by Claisen-Schmidt condensation reaction for substituted benzaldehydes with N-(4H-1, 2, 4-triazol-4-yl) acetamide under basic conditions. The synthesized compounds were characterized by detailed spectral analysis of 1 H NMR, MS, and IR spectroscopy. The synthesized Chalcone derivatives 3a, 3b and 3c were screened for their anti-fungal activity by using agar diffusion method against Aspergillus niger and Penicillium notatum. All the compounds have shown significant anti-fungal activity. The compound 3a has shown good anti-fungal activity compared to other compounds and flucanazolewas taken as standard against both Aspergillus niger and Penicillium notatum.