A rapid, simple, selective, sensitive, precise and specific UV Spectrophotometric method has been developed for the determination of Domperidone in API and pharmaceutical dosage form. Domperidone standard solution was scanned in the UV range (200-400nm) in 1cm quartz cell in a double beam UV Spectrophotometer. The spectrophotometric detection was carried out at an absorption maximum of 288 nm using Methanol as a solvent. The method was validated for specificity, linearity, accuracy, precision, robustness and ruggedness. The detector response for the Domperidone was linear over the selected concentration range 5-25µg /ml with a correlation coefficient of 0.999 and equation for the regression curve was found to be y=0.0297x+0.115. The accuracy was between 99-101%. The precision (%RSD) among six samples preparation was 0.308%. The LOD and LOQ are 0.045 and 0.1µg /ml respectively. Statistical analysis proved that the methods are repeatable and specific for the determination of the said drug. These methods can be adopted in the routine assay analysis of Domperidone in API and pharmaceutical dosage form.

A rapid, simple, selective, sensitive, precise and specific UV Spectrophotometric method has been developed for the determination of Chlorthalidone in bulk and (200-400nm) in 1cm quartz cell in a double beam UV Spectrophotometer. The spectrophotometric detection was carried out at an absorption maximum of 227 nm using Methanol as solvent. The detector response for the Chlorthalidone was linear over the selected concentration range 2-10µg /ml with a correlation coefficient of 0.999 and equation for the regression curve was found to be y=0.071x+0.182. The accuracy was between 97-100%. The precision (%RSD) among six samples preparation was 0.160%. The LOD and LOQ was 0.251 and 0.3200µg/ml respectively. Statistical analysis proved that the methods are repeatable and specific for the determination of the said drug. These methods can be adopted in the routine assay analysis of Chlorthalidonein API and pharmaceutical dosage form.

The purpose of this study was to enhance the dissolution rate of poorly water soluble drug of Ketoconazole by preparing spherical agglomerates by Quassi emulsion solvent diffusion technique using chloroform, water, and methanol as bridging liquid and to illustrate the effect of different polymers on the solubility and dissolution rate of Ketoconazole. Polyvinyl Pyrrolidone (PVP K30) was used in spherical agglomeration process. The formulation was done by 32-full factorial design. Stirring speed and the concentration of PVP K30 were used as the variable factors in the applied design. Fourier Transform Infra-Red spectroscopy was used to examine the drug-excipient compatibility. Prepared formulations were evaluated for micromeretic properties and dissolution rate. The spherical agglomerates have lower micrometric properties compared to pure drug. The agglomerates exhibited good compressibility and packability characteristics. The spherical agglomerates with different polymers exhibited increasing in the saturation solubility and dissolution rate.

A simple, rapid, precise and highly selective spectrophotometric method was developed for simultaneous estimation of Alprazolam and Propranolol hydrochloride in pure as well as tablet dosage form. The simultaneous equation method is based on measurement of absorbance at 263 nm and 289 nm as two wavelengths selected for quantification of Alprazolam and Propranolol Hydrochloride using 0.1 N HCl as a solvent. The method was validated for specificity, linearity, accuracy, precision, robustness and ruggedness. A double-beam shimadzu UV-visible spectrophotometer, 1800 with a pair of 1 cm matched quartz cells was used to measure the absorbance of the solutions in developed method. The method was validated as per ICH guidelines. Linearity ranges from 5-25 µg/ml for Alprazolam and 10-50 µg/ml for Propranolol hydrochloride of the drugs. % RSD calculated was less than equal to 2 which indicates accuracy and reproducibility of the method. Recovery study indicates that these drugs could be quantified simultaneously without interference of excipient present in formulation. The developed UV spectroscopic method is suitable for the analysis of ALP and PRP in combined dosage form. The accuracy was found between 98-100% for ALP and 99-100% for PRP respectively. The precision (%RSD) was found to be 0.308 for ALP and 0.875 for PRP respectively. The LOD was found to be 0.041µg/ml for ALP and 0.094µg/ml for PRP respectively.

A simple, precise, accurate, economical and reliable UV spectrophotometric method has been developed for the estimation of Olopatadine hydrochloride in bulk and its tablet dosage form. The drug shows maximum absorption (λmax) at 231 nm in methanol and obeys Beer’s law in the concentration range of 3-15 µg/ml with correlation coefficient (R2=0.999). The accuracy was found to be 97.2-99.6%. Limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.047µg/ml and 0.144µg/ml respectively. The relative standard deviation was found to be <2.0% in all cases. The proposed spectrophotometric method was validated as per ICH Q2 (R1) guidelines. Statistical analysis proved that the method is repeatable and specific for the determination of the said drug. The proposed method can be used for the reliable quantification of Olopatadine hydrochloride in bulk form and routine analysis of pharmaceutical formulations.