Background: Cefotaxime (CTX) is a third generation cephalosporin antibiotic. Sulbactum (SBT) is semi-synthetic parenteral penicillin with a broad spectrum of antibacterial activity. The combination of Cefotaxime and Salbactum is more effective for the treatment of moderate to severe infections than single drug therapy alone. There are so many combinations of sulbactum and other cephalosporin on which RP-HPLC method was developed. Till date no HPLC method is available for simultaneous estimation of sulbactum and cefotaxime in combined dosage form. Objectives: The present work involves the development of new and validated RP-HPLC method for simultaneous estimation of CTX and SBT in reconstituted solids as combined dosage form. Material and Methods: The simultaneous estimation of Cefotaxime and Sulbactum has been done by RP-HPLC on Hypersil C-18 (Gold) column (250 mm x 4.6 mm) using 10mM Potassium dihydrogen phosphate buffer (pH- 5.1): Acetonitrile (90:10 v/v) as a mobile phase at the flow rate of 1.0 ml/min. and quantified at 228 nm using UV detector. Results and Discussion: Cefotaxime and Sulbactum were satisfactorily resolved with Retention time (Rt) of 8.56 min. and 4.00 min. respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (Cefotaxime 20-140 μg/ml and Sulbactum 10-70 μg/ml respectively), precision intra-day and inter-day RSD values were always less than 2 for them, accuracy (99.66% ±3% for Cefotaxime and 99.51 ±2% for Sulbactum) and specificity, in accordance with ICH guidelines. Conclusion: The statistically validated results indicate that the proposed new method has good accuracy and precision. Thus new HPLC method has been successfully applied for the simultaneous estimation of Cefotaxime and Sulbactum in combined dosage form as reconstituted solids.

A simple accurate and reproducible stability indicating RP-HPLC method with UV-visible spectrophotometer was developed for the estimation of degraded products of Nepafenac. A novel isocratic RP-HPLC method has been developed using SHISEIDO CAP CELL PAK C18 column (4.6mm I.D× 250mm, 5µm) with the mobile phase composition of Acetonitrile: phosphate buffer (pH -4) [60:40] at flow rate 1ml/min, with column temperature 250 C and effluent was detected at 240nm. The method showed linearity over the range 0.1-4µg/ml with correlation coefficient of 0.9991. Nepafenac drug was subjected to acidic, alkaline, neutral, oxidative, thermal and photolytic stress conditions. Nepafenac was found to be degrading significantly in alkaline, acidic and oxidative stress conditions. Finally the drug was found stable in thermal, neutral and photolytic stress conditions. The developed method was validated according to ICH guidelines with respect to linearity, specificity, accurate, precise and robustness.

A simple and accurate reversed phase HPLC method for simultaneous estimation of Syrian cough syrup containing Bromhexine Hydrochloride, Terbutaline Sulfate, and Guaiphenesin was developed. Separations were carried out on Column: C18(1005) (25X4.6mm, with precolumn). The mobile phase was methanol: buffer (600:400 v/v). The elution of the analytes was achieved in less than 10 min with a flow rate of 1.5 ml/min. Detection was by using UV absorbance at a wavelength of 276 nm. Different analytical performance parameters such as linearity, precision, accuracy, and robustness were determined and found in the acceptance range of American pharmacopoeia (USP34).

Propolis is a resinous Bee hive product and has been long used in folk medicine of different nations as early as 3000 BC. It consists of exudates from plants mixed with bees¬wax. The present study was carried out to identify the phytocomponents present in the methanolic extract of the propolis of honey bee Apismellifera by GC-MS analysis. From the GC-MS results thirteen compounds were identified as major constituents, they are Ethylhexanol, 3 ethyl 3 methylheptane, Dodecane, 1,1 dimethylethyl, Tetradecane, 4,6 dimethyl, Tetracosane, Diethyl phthalate, Dibutylpbhthalate, Dibutylpbhthalate, Hexadecanoic acid, Octadecenoic acid, 1,2 benezenedicarboxylic acid, Hexatricontane. These different active phytochemicals have been found to possess a wide range of biological activities, which may help in the protection against incurable diseases.

A simple, specific, precise, accurate, rapid and reproducible efficient reversed phase HPLC method with PDA detector has been developed and validation for simultaneous estimation of fluticasone (FLP) and azelastine (AZH) in pharmaceutical dosage form. Chromatography was performed on a 150mm X 4.6mm, 5μm particle size, Altima C18 column with a 62: 33:5 v/v/v mixture of buffer pH4.0: acetonitrile: methanol as a mobile phase. The detection of the combined dosage form was carried out at 235nm and flow rate employed was 1.0ml/min. The retention times were 2.1±0.3 and 3.1±0.3 min for fluticasone and azelastine respectively. Linear was established in the concentration range of 10.0 to 75.0μg/ml for FLP and 27.4 to 205.5μg/ml for AZH with a correlation coefficient of both drugs for found to be 0.999. The recoveries obtained were 99.80 -100.12% for FLP and 99.68 -100.26% for AZH. Similarly the %RSD value for precision was also found to be within the acceptable limit. The method was validated according to international conference of harmonization guidelines in terms of accuracy, precision, specificity, robustness, linearity and other aspects of analytical validation. The results of the analysis were validated statistically and recovery studies confirmed the accuracy and precision of the proposed method. Developed method was rapid and convenient which could be successfully applied for the routine control of both the component.

Two simple and sensitive spectrophotometric methods are described for determination of irbesartan, losartan, hydrochorothiazide and atenolol in bulk and tablet forms. Method (I) depends on formation of the colored chromogen by condensation reaction between irbesartan, losartan and atenolol and vanillin in acidic conditions and the product was measured at λmax 546,552 and 560 nm for irbesartan, losartan and atenolol, respectively. Under the indicated conditions, this method was linear over the concentration range of 40-240 µg/ml, 80-240and 40-200 µg/ml for irbesartan, losartan and atenolol, respectively. In method (II), 1, 2-Naphthoquinone-4-sulphonate sodium reacts with irbesartan, losartan and hydrochlorothiazide through nucleophilic substitution reaction producing orange colored product in alkaline medium showing maximum absorption at λmax 465 nm for irbesartan, losartan and hydrochlorothiazide where the method was linear over the concentration range of 1-6 µg/ml, 0.2-1 µg/ml and 0.25-1.25 µg/ml for irbesartan, losartan and hydrochlorothiazide, respectively. The methods were statistically applied for the determination of drugs in both bulk and tablet forms. Results were compared with reference methods and no statistically difference was obtained.

Randialic acid B found in Ayurvedic medicinal plant Randia spinosa (Poir.) Rubiaceae exhibit aphrodisiac, emetic, abortifacient, antipyretic, carminative, alexiteric and cures abscesses, ulcers, inflammations, tumors, skin-diseases, piles. Occurance of Randialic acid Bin other plants is so far not reported. Randialic acid B was taken up for chromatographic analysis. Multiphase solvent extraction of stem bark of R. sapinosa was done to isolaterandialic acid from stem bark of Randia spinosa. Crystallization of Randialic acid B was confirmed by melting point 256oCIdentification was done by TLC method. The objective of this work was to determine the standard HPLC chromatograms Randialic acid B found in bark of Randia spinosa. This HPLC fingerprint of Randialic acid B could be used as benchmarks for comparison during the qualitative and quantitative analysis of Randialic acid B present in any plant sample.

An isocratic HPLC method had been developed for rapid simultaneous separation and determination of carvedilol and atorvastatin calcium in pure form and pharmaceutical preparation. Separation was carried out on a Hypersil gold C18 (15um, 100x4.6mm) column. Mobile phase composed of methanol, acetonitrile and 0.1% ortho phosphoric acid in the ratio of 40:45:15(v/v/v) with 0.6 ml per min flow rate and detection was at 254 nm. Linearity was obtained in the concentration range of 1-90 (μg. mL-1) for both drugs. The method was applied for the determination of drugs in both bulk and pharmaceutical formulation and was validated when obtained results were compared with reference methods.