Extracts from Ligustrum lucidum Ait leaves have been used in medicine for centuries, a fast and effective method to detect phytochemicals in Ligustrum lucidum Ait leaves has important significance for pharmaceutical research. In this study, leaf spray-MS was employed to directly fingerprint the phytochemicals in Ligustrum lucidum Ait leaves. By adding 10 μL methanol on the surface of the V-shaped sample, charged droplets containing a variety of phytochemicals extracted from the leaf tissue such as sugars, alkaloids and flavonoids were produced with high voltage applied on the sample. The metabolism information during the growth of Ligustrum lucidum Ait leaves were preliminary investigated by comparing the phytochemicals patterns of the half-growth and the full-growth leaves. Our experimental results provide useful information for the pharmaceutical research and clinical applications of Ligustrum lucidum Ait leaf.

There is a steady need for novel antibacterial compounds from plants. Stevia rebaudiana (SR) is an indigenous plant in Iran, which the plant has been used as an antioxidant, antifungal, antiviral, and antiflammatory agent in Iran. As we know, there is no documented proof on antibacterial activities of SR alcoholic extract against Escherichia coli O157: H7 (EC) in west of Iran. As a screen test to discover antibacterial properties of the extract, agar disk and agar well diffusion methods were employed. Macro broth tube test was performed to specify MIC. The results of agar disk and agar well diffusion tests represents SR have prevented the growth of EC. Also in many of samples by increasing the concentration of SR, the inhibition zone increased. The MIC and MBC values were 0.031 g/ml for SR. Thus, the research showed the antibacterial effects of the ethnomedicinal herb on EC. We believe that the article provide support to the antibacterial activities of the extract.

A simple, accurate and precise reverse phase HPLC method validated for the determination of RIZATRIPTAN Tablet dosage form. Chromatography was carried on inertsilods 3v 5µm column using a mixture of PHOSPHATE BUFFER and acetonitrile, pH 7.8(in the ratio 70:3o v/v) as the mobile phase at a flow rate of 1.0ml /min with detection at 235 nm by ultra violet detector i.e. incorporated in HPLC. The retention time of the drug was found to be 3.360min. The method validation proofs were carried out as per the ICH guidelines. The developed method was validated for linearity over a range of 30μg/ml to 70μg/ml, with a correlation coefficient of 0.998, which shows the method is quite linear. Further precision, ruggedness, accuracy were validated. The % RSD for system precision was observed to be Less Than 2, the average recovery of 100.0% indicates the capability of the method, and finally no significant differences in % RSD values with respective Retention time prove the robustness of the method. As per ICH guidelines, method validation results are in good agreement. The proposed approach is effective and can be applied for the tablet dosage form estimation of Rizatriptan in tablet dosage form.

Breast cancer is one of the most leading cause in the cancer deaths. Nine out of ten women who develop breast cancer do not have a family history of the disease. Survival rate five years after diagnosis is 88%. Breast cancer is of Ductal carcinoma in-situ and invasive. Breast cancer is caused by Genetic mutations-BRCA1 and BRCA2, Environmental exposure to UV radiation, air pollution. Symptoms may include breast lump, bloody discharge from the nipple, dimpling, Redness. Numerous growth factors that also play a role in a tumor development or secreted by breast cancer cells that are Autocrine growth factors and Paracrine growth factors. Diagnosis of breast cancer include: Breast exam, Mammogram, ultrasound, Biopsy. The four main treatments for breast cancer are radiotherapy, hormone therapy, chemotherapy and surgery. A new research identified molecular markers of tamoxifen resistance, an anti-oestrogen drug that is one of the most successful treatments available for breast cancer.

The present work proposed precise, accurate and validated HPLC and UV-spectrophotometric methods for estimation of Teneligliptin from its tablet dosage form. The UV-spectrophotometric estimation includes Calibration curve, Area under curve (AUC) and First order derivative method based on measurement of absorbance at a selected wavelengths using UV-visible spectrophotometer with 1cm matched quartz cell and distilled water as a solvent. All UV-spectrophotometric methods obeyed Beer’s-lambert’s law in the concentration range of 10-70μg/mL, with correlation coefficient value less than 1. The chromatographic separation was achieved by isocratic mode with a mixture of methanol: phosphate buffer (pH 7.2) in the ratio of 70:30 v/v as the mobile phase using Shodex C18 column as stationary phase at flow rate of 1mL/min and detection wavelength of 244nm. The retention time was found to be 5.753min. The percent amount of drug estimated by all developed methods was nearly 100%, found to be in good agreement with label claim of marketed tablet formulation. The recovery study was carried out at five different levels and results were found to be satisfactory. The validation parameters like accuracy, precision, ruggedness, linearity and range were studied for all the developed methods and were found to be within limits. Stress testing under various conditions such as pH (acid/base), temperature, light, oxidation, humidity was carried out and % undegraded drug was calculated.