An isocratic, reversed phase-liquid-chromatographicmethod was developed for the quantitative determination of Azithromycin and Levofloxacin in combined-tablet dosage form. A water Symmetry ACE 5Rp18, (150*4.6*5µ) column with mobile phase containing 0.1% ortho phosphoric acid: Methanol in the ratio of (60:40v/v) was used. The flow rate was 1.0mL/min, column temperature was 30°C and effluents were monitored at 280nm. The retention times of Azithromycin and Levofloxacin were 3.623 min and 2.401min, respectively. The correlation coefficient for Azithromycin and Levofloxacin were found to be 0.99 and 0.99, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recoveryof Azithromycin and Levofloxacin in formulations was found to be inthe range of 97-103% and 97-103% respectively confirms the non-interferences of the excipients in the formulation. Due to its simplicity, rapidness and high precision. The method was successfully applied to the estimation of Azithromycin and Levofloxacin in combined dosage form.

An isocratic RP-HPLC method has been developed and validated for rapid simultaneous separation and determination of mixture of secnidazole, omeprazole and amoxicillin in their pure forms and pharmaceutical preparations. The separation was carried out on ACE 5 C 18 (25 cm x 4.6 mm) column using a mobile phase of acetonitrile: potassium dihydrogen phosphate buffer (pH 7.5) (60: 40, v/v). The flow rate was 1.5 ml/min and the detection was set at 230 nm. The proposed method was successfully applied to the estimation of the cited drugs in their dosage forms.

Despite the great development of organic synthesis, currently 25% of prescribed drugs worldwide are still derived from plant source, showing that plant species are still an important source of new drugs for diseases that continue to lack a cure, such as cancer. The present study was carried out to evaluate the anticancer activity of aqueous and ethanolic extract of the whole plant of Dipteracanthus prostratus(AEDP, EEDP respectively) in mice. The anticancer activities of AEDP, EEDP were studied against Ehrlich ascities carcinoma (EAC) in mice. It was revealed that administration of AEDP, EEDP of whole plant of Dipteracanthus prostratus exhibited ability on reduction significantly in tumor volume, viable cell count, while increasing significantly the non viable tumor cells count compared to EAC control group. It was found that the mean survival time and percentage of life span inmice were increased in extracts treated groups. These resultssuggest that the extract of whole plant of Dipteracanthus prostratus exhibits potential anticancer activities.

Triazole ligands and their metal complexes are very important in medicinal and pharmaceutical fields because of their wide spectrum of biological activities. This reviewdescribes biological activity on triazole containing metal complexes. Triazoles are regarded as a promising class of bioactive heterocyclic compounds that exhibit a range of biological activities like anti-microbial, anti-viral, anti-diabetic, anti-cancer activity, anti-oxidant, anti-proliferative, anti-HIV, anti-convulsant, anti-inflammatory, etc. These promising results are encouraging further for inorganic as well as bio-inorganic chemists.

A novel stability-indicating high pressure liquid chromatographic(HPLC) method was developed and validated for quantitative determination of Nimesulide (NIM) in bulk drug and in tablets. Isocratic, HPLC method, using a C18 reversed phasecolumn with mobile phase 50% acetonitrile, 50% aqueous contained 0.05% orthophosphoric acid as mobile phase adjust PH at 2.8 and temperature at40°C. The proposed method was investigated to separate the drug from its stress degradation products. The flow rate was 1.5 ml/min, column oven temperature was ambient and detection of column effluent was performed at 220 nm. NIM was subjected to the stress conditions of hydrolysis (acid and base), oxidation and heat degradation. Stress degraded samples were analyzed by the developed procedure. The analyte was well separated from its degradants. The described method showed excellent linearity over a range of 1-200 µg/ml. The determination coefficient for NIM was 0.9998. Limit of detection for NIM was 0.98 µg/ml and limit of quantification was 2.97 µg/ml respectively. Degradation of NIM was observed in acid, base and in 35% H2O2 conditions only. The drug was found to be stable in the other stress conditions attempted. The percentage recovery of NIM ranged from (99.72% to 100.1176%) in tablets. The developed method was validated with respect to the linearity, accuracy (recovery), precision, specificity and robustness. The forced degradation studies proved stability indicating power of the method.

Simple, rapid, and sensitive direct spectrophotometric methods for the determination of nimesulide (NIM) in pure form and pharmaceutical formulations have been developed. The methods depend on the formation of colored ion-pair complexes between NIM and three different reagents, bromocresol green (BCG), bromocresol purple (BCP) and brilliant blue G (BBG) in Britton-Robinson (B-R) universal buffer solutions. The colored complexes were measured directly exhibiting λ _max at (554, 437 and 643 nm) for BBG, BCP and BCG respectively. The analytical parameters and their effects were investigated. The ion-pair complexes are intensely colored and very stable at room temperature. The calibration graphs were linearover the concentration range of 2 - 12 μg/mL for BCP, 2-14 μg/mL for BCG and 2-13 μg/mL BBG. The stoichiometryof the reaction was found to be 1: 1 in all cases. The proposedmethods were successfully extended to pharmaceutical preparations in tablet dosage form. 

Delavirdine has been approved by the U.S. food and drug administration for its application in the treatment of AIDS and AIDS related opportunistic infections. As its efficacy is lower than other NNRTIs therefore, U.S. Department of Health and Human Services has recommended its use not as a part of initial therapy but in combination with other drugs. To circumvent this therapeutic difficulty a search for new delavirdine analogues with enhanced activity was needed to be perused. It has been observed that heterocycles that incorporates isoxazole and pyrazole in their molecules exhibit wide range of biological properties such as antibiotic, anticancer, antiviral, and anti-HIV activity. In view of this, it was considered of interest to incorporate isoxazole and pyrazole in the molecular framework of delavirdine. In this communication, we report the preliminary results of our study which was directed to incorporate these biologically active heterocyclic scaffolds in the molecular framework of delavirdine molecule.

Oxoketene dithio acetals (4) reacted with cyclic secondary amine namly N-methyl piperazine to produce oxoketene N,S-aminal (5) in good yield. This research work explores the synthetic utility of oxoketene-N, S-acetals derived from oxoketenedithioacetals in the synthesis of a wide variety of heterocyclic compounds of potential biological interest.

A new simple, precise, rapid and accurate reverse phase high performance liquid chromatographic method had been developed for the simultaneous estimation of nimorazole (NIM) and ofloxacin (ofx) in pure and its pharmaceutical dosage form. The chromatographic separation was achieved on a phenomenex luna C18, 250 x 4.6 mm, 5μm particle size column was used with PDA detector by using mobile phase containing mixture of 0.02M Potassium dihydrogen orthophosphate (KH₂PO4) buffer : acetonitrile (95:5 % v/v pH 6.92) was used. . The flow rate was 1 ml / min and effluents were monitored at 260 nm. Chromatogram showed two main peaks corresponding to ofloxacin and nimorazole at retention times 3.96 and 11.55 min respectively. The method was linear over the concentration range of 50-250μg/ml for nimorazole and 20-100 μg/ml for ofloxacin respectively. The developed method was validated in accordance to ICH guidelines. 

Four simple sensitive and accurate spectrophotometric methods were developed  for determination of  Albendazole in pure and  in tablet forms. The method (I) depends on reaction of drug and  Vanillin reagent in acidic condition, the red colored product was measured l_max. 562 nm.Beer’s lawwas obeyed in the range of 10-110 µgml^-1. In method (II) 1,2-Naphthoquinone-4-sulphonate sodium  reacts in alkaline mediam  through nucleophilic substitution reaction producing an orange-brown colored product showing maximum absorption at 482 nm. Beer’s lawwas obeyed in the range of 0.2-3µgml^-1.Method( III) charge transfer complex was formed between Albendazole and Tetracyanoethylene measured  at l_max. 395 nm . Beer’s lawwas obeyed in the range of 0.1-1µgml^-1.Method (IV) based on bromination-oxidation reaction using bromate-bromide mixture with  rhodamine B and  thymol blue as reagents and measuring the absorbance of the unbleached dye at 555 nm and 545 nm.  Beer’s lawwas obeyed in the range 1.5-4.5µgml^-1 and 2-16 µgml^-1 respectively.