The aim of the present study was to extract lecithin from egg yolk by precipitating it with acetone followed by centrifugation to separate supernatant liquid from the precipitate and then evaporate solvent with the help of lyophilizer. Egg lecithin was standardized by TLC analysis and FTIR spectroscopy. Egg lecithin was successfully isolated in a very economical way from egg yolk and all the parameters were evaluated and found within the specified limit. FTIR and chromatographic analysis shows the presence of ester linkage, alkane and hydroxyl group.

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of Eperisone hydrochloride in bulk and pharmaceutical dosage form. The stationary phase used was silica gel precoated aluminum plate 60F254 plates. The mobile phase used was a mixture of ethyl acetate: methanol: toluene (4:3:3, v/v/v). The detection of spots was carried out at 272 nm. The method was validated in terms of specificity, accuracy, linearity, precision and accuracy. The calibration curve was found to be linear between 100-700 ng/band. The proposed method can be successfully used to determine the drug Eperisone hydrochloride in bulk and pharmaceutical formulation.

A simple, economic, sensitive, precise and accurate UV spectroscopic method was developed and validated for determination of Lopinavir in bulk and tablet dosage form. Adequate drug solubility and maximum assay sensitivity was found in Acetonitrile at 220nm. Calibration graph constructed at 220 nm was linear in concentration range of 10-30 μg/ml with correlation coefficient of 0.999. The method was validated as per ICH guidelines in terms of linearity (within 10-30 µg/ml), accuracy (% recovery), precision (inter-day and intraday), specificity and robustness. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.130µg/ml and 0.394µg/ml respectively. Therefore, the proposed method is suitable and can be adopted for the determination of Lopinavir from pharmaceutical dosage form in routine quality control analysis.

A simple, precise, accurate, rapid and sensitive Reverse-Phase High Performance Liquid Chromatography method for the estimation of Escitalopram in tablet dosage form was developed and validated. Detection was carried out at 240 nm. The mobile phase methanol: water (90:10 v/v) pH 3.0 is adjusted with formic acid at a flow rate of 1.0 ml/min. The retention time of Escitalopram was found to be 2.80 min. The standard curve was linear (R2>0.9950) over the concentration range of 2-20 µg/ml. The analytical method developed was validated as per ICH guidelines. The selectivity, robust and reliable as accuracy, precision, recovery and other validation parameters were within the limits as specified by the guidelines. The peaks were symmetrical in nature with acceptable tailing factor. The method can be very useful for the therapeutic drug monitoring (TDM), in bioequivalence studies, for pharmacokinetics study and also in toxicology and biomedical investigations.

A simple, precise, accurate, economical and reliable UV spectrophotometric method has been developed for the estimation of Cefixime trihydrate in bulk and its tablet dosage form. The drug shows maximum absorption (λmax) at 288 nm in methanol and obeys Beer’s law in the concentration range of 2-10 µg/ml with correlation coefficient (R2=0.999). The accuracy was found to be 98-99%. Limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.042µg/ml and 0.096µg/ml respectively. The relative standard deviation was found to be <2.0%in all cases. The proposed spectrophotometric method was validated as per ICH Q2 (R1) guidelines. Statistical analysis proved that the method is repeatable and specific for the determination of the said drug. The proposed method can be used for the reliable quantification of cefixime trihydrate in bulk form and routine analysis of pharmaceutical formulations.