Many extraneous strategies of high-performance liquid chromatographic method development are used today. This review reports a strategy for the systematic development of High-performance liquid chromatographic (HPLC) methods. HPLC is an analytical tool which is effective to detect, separate and quantify the drug, its several impurities and drug related degradants that can form on synthesis or storage. It involves the comprehension of chemistry of drug substance and facilitates the development of analytical method. A number of chromatographic parameters were evaluated in command to optimize the method. An actual mobile phase, column temperature, column, wavelength and gradient must be expert that affords appropriate compatibility and stability of drug as well as degradants and adulteration.Most of the drugs in numerous component dosage forms can be studied by HPLC method because of some advantages like rapidity, specificity, recovery, precision and ease of automation in this method. Validation of HPLC method as per ICH Guidelines protects all the performance characteristics of validation, like Accuracy, precision, specificity, linearity, range and limit of detection, limit of quantification, robustness and system suitability testing.

Glucagon is a peptide hormone consisting of 29 amino acids and is useful for maintenance of normal glycemia in blood1. Generally, peptide/proteins are highly sensitive to digestive enzymes present in the body, hence their administration is usually restricted to injection or nasal administration. To achieve enough stability of Glucagon, a suitable excipient needs to be chosen that could stabilize the peptide during freeze drying process and long-term storage as a dried formulation. Usually, Lactose is used as a stabilizer for freeze-dried formulations of glucagon and upon storage Glucagon will react with lactose via Maillard reaction and lead to formation of impurities. In our study, we prepared Glucagon lactose impurity, Glucagon di lactose impurity, and further purified them by using Preparative HPLC.

Bioequivalence study of azithromycin of three brands (local, regional and national) was investigated by this new In-vitro densitometric method. The proposed method depends on measuring the optical density of the inhibited zone area of Klebsiella pneumonia and Staphylococcus aureus caused by the incubation of azithromycin using highly resolution digital camera and Image J software. Good correlations were obtained between the inhibited zone area and the antibiotics concentrations (0.993-0.994). The limits of detection and limits of quantitation were 2.23, 6.69 and 2.22,6.66µg/mL with Klebsiella pneumonia and Staphylococcus aureus; respectively. The proposed method was validated according to US-Food and drug administration (FDA) guidance for bioanalytical method validation and USP 31 guidelines. The bioequivalence study revealed that all the investigated dosage forms brands are bioequivalent.

Coronaviruses are one of the transmissible viruses that are mostly reliable for respiratory, intestinal and urogenital tract infections. Various researchers demonstrated that the main protease (MPRO) protein might be an important drug target for SARS-CoV-2. The treatment of illnesses by the oral tradition have long been associated with Natural herbal remedies (NHRs). Modern medicine has potential effects, thanks to traditional medicine, the effectiveness of which derives from medicinal plants including with herbs and shrubs. Objective of this study is to confirm the ingredient of the natural origin compounds (NOCs) which have a potential of anti-viral effect (AVE) and can prevent the humans from SARS-CoV-2 infection. We are interested to figure out the interaction study between the molecules of natural origin compound and the protein of SARS-CoV-2 through molecular docking. The inhibition of Coronavirus (nCoV-2019) main protease enzyme is an important target of our study. All the compounds from Curcuma longa L. (Zingiberaceae family) had been screened for the inhibition of MPRO protein through in silico methods. From molecular docking report the results we obtained that is out of 235 molecules of natural origin the derivative of Curcumin are one of the best compounds determined through molecular docking and hydrogen bonding with interaction are proposed as the may be novel inhibitor for the SARS-CoV-2 main protease. We demonstrate using Swiss ADME online server tools that all sixteen molecules have better “drug-likeness” than control and does not violate any Ghose, Lipinski, Egan, Veber or Muegge rules. Importantly, all sixteen compounds may be more potent than chloroquine in treatment of COVID-19 according to Molecular docking, interactions and ADME Properties.

Hydrochlorothiazide and chlorthiazide are high potent diuretics used in treatment of hypertension. In recent research trends shows that chlorthiazide derivatives are used in osteoporosis and also has antimicrobial activity against various bacterial species, In this view a series of Chlorthiazide Derivatives were designed, synthesized six compounds by condensing chloroacetyl chloride with chlorthiazide by using ethanol as a solvent refluxed at 60c temperature formed compound is taken as intermediate further refluxed and condensed with Aromatic amines there by formation of substituted chlorthiazide derivatives, (Ctd-1, Ctd-2, Ctd-3, Ctd-4, Ctd-5, Ctd-6) and confirmed by the physical and chemical properties, IR, NMR spectra’s. Screened for Antibacterial activity.

Densities ( - Phenyl-2- bromobenzohydroxamic acid (PNBHA) in dimethylsulphoxide (DMSO) have been measured at temperatures (298.15, 303.15, 308.15 and 313.15K) under atmospheric pressure. Apparent molar volume (?v) and partial molar volume (? 0 v ) and Excess Molar Volumes have been calculated from experimental values of densities. These parameters have been used to discuss the solute-solvent interactions.