A simple, rapid and precise reverse phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for estimation of ceftaroline fosamil in bulk drug and pharmaceutical dosage forms. The analysis was carried out using C₁₈ - Octa decyl silane -250cm x4.6mm i.d, 5µ. The separation was carried out using a mobile phase containing Buffer: Acetonitrile: Methanol (40:30:30), was pumped at a flow rate of 1.0mL/min with UV-detection at 242nm. The present study was carried out to develop a simple, sensitive, accurate and more precise UV and RP-HPLC methods for the estimation of ceftaroline fosamil in bulk and pharmaceutical dosage forms. The % purity of was calculated by using both methods and it was found to be 100.02% and 99.63%, respectively and correlation coefficient of 0.999. The proposed UV and HPLC methods can be applied for the routine quality control analysis of ceftaroline fosamil in bulk and IV Injection forms.

Cosmeceuticals are the advanced addition to the pharmaceutical industry; they are cosmetic products with drug-like activities. The word cosmeceuticals was coined by Klingman. Marine resources exist in vast numbers and they show enormous diversity. As a result, there are lots of possible applications for marine constituents of interest in the cosmetic industry, whether as active ingredients, excipients or additives. The active agents from the marine source used for the preparation of cosmeceuticals are sea weeds, marine fishes, phytoplanktons, sponges, corals, sea mammals, fungi and bacteria and mollusces. Marine ingredients used for the preparation of cosmetics having various properties such as anti aging, skin whitening, moisturizing, de-pigmentation and antioxidant activity etc. Lot of cosmeceuticals which contains marine ingredients is available in the market also. The potential of marine ingredients is a fascination of study by researchers for the last years.

Simple, precise and accurate area under curve spectroscopic method has been developed and validated for the estimation of Rosuvastatin calcium in bulk and Pharmaceutical dosage form. The drug shows maximum absorption (λ_max) at 242nm in Acetonitrile solution and Area under Curve [AUC] in absorption spectra were measured between the wavelength range 237 to 247nm which obeys Beer’s law in the concentration range of 3-18µg/ml. The linearity study was carried out and regression coefficient was found to be 0.9998 and it has showed good linearity, precision during this concentration range. The % recovery was found to be 98.94-101.25. The LOD and LOQ were found to be 0.037 and 0.114µg/ml. The % relative standard deviation was found to be less than 2. According to ICH guidelines the method has been validated for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for routine quantitation of Rosuvastatin calciumin bulk and pharmaceutical dosage form.

Simple, precise and accurate zero order derivative spectroscopic method has been developed and validated for the estimation of Sitagliptin in bulk and Pharmaceutical dosage form. The drug shows maximum absorption (λ_max) at 267nm in 0.1N sulphuric acid solution and obeys Beer’s law in the concentration range of 10-50µg/ml. The linearity studywas carried out and regression coefficient was found to be 0.9999 and it has showed good linearity, precision during this concentration range. The % recovery was found to be 98.49-99.98. The LOD and LOQ were found to be 0.251 and 0.761µg/ml. The % relative standard deviation wasfoundto be less than 2.As per ICH guidelines the method has been validated for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for reliable quantification of Sitagliptin in bulkand pharmaceutical dosage form.

Simple, precise and accurate area under curve spectroscopic method has been developed and validated for the estimation of Ursodiolin bulk and pharmaceutical dosage form. The drug shows maximum absorption (λ_max) at 240nm in Methanol: Acetonitrile: O-phosphoric acid (40:40:20) solution and Area under Curve [AUC] in absorption spectra were measured between the wavelength range 235 to 245nm which obeys Beer’s law in the concentration range of 2-10µg/ml. The linearity study was carried out and regression coefficient was found to be 0.9999 and it has showed good linearity, precision during this concentration range. The % recovery was found to be 98.25-99.55. The LOD and LOQ were found to be 0.154 and 0.469µg/ml. The percentage relative standard deviation is found to be less than 2. According to ICH guidelines the method have been validated for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for routine quantitation of Ursodiolin bulk and pharmaceutical dosage form.

The objective of the current study was to determine the pharmacognostical parameters for the plant Decalepis hamiltonii's tuberous roots (Asclepiadaceae). The Root is traditionally used for blood disorders, diabetes, gout, urticaria, haemorrhage, thirst, and depurative purposes. To fully harness this folk herb's therapeutic potential, an effort has been made to correctly identify it. The morphoanatomy of tuberous roots, in conjunction with microscopic linear measurements, WHO-recommended physico-chemical determinations, and genuine phytochemical procedures, are the key diagnostic characters that have been carried out to help with the thorough pharmacognostical evaluation of the plant. The parameters discussed in this paper could be suggested as the benchmarks for determining the legitimacy of Decalepis hamiltonii. This research aids in separating this medication from its other species.