Objectives: this study intended to evaluate the cytotoxicity of Biodentine and MTA materials to fibroblast cell lines. Materials and Methods: MRC-5 and BHK-21 fibroblast cell lines were incubated for 1 day in extracts from immersion of MTA and Biodentine materials in culture medium for either 1, 3, or 7 days as well as directly with fresh set materials immersed in culture medium. Fibroblasts cultured in DMEM were used as a control group. Cytotoxicity was evaluated using MTT assay. The data of cell cytotoxicity were analyzed statistically by two ways and one-way analysis of variance at a significance level of P < 0.05. Results: MTT viability assay revealed that MTA preserved cell viability better than Biodentine. It also revealed that 7th day extract significantly showed higher number of viable cells than 1stand 3rd days extracts (P < .05). Conclusion: Within the limitation of this study, Biodentine revealed more cytotoxicity than MTA, however, both materials exhibited biocompatibility and can be used in contact with living cells.

Objectives: Synthesis and in vitro anti-coagulant activity of some novel coumarin derivatives. The need for anti-coagulant drugs is because the Stroke, heart attack, and pulmonary embolism are extremely common and often fatal conditions. Methods: Coumarin derivatives were prepared by the condensation of 4-hydroxy coumarin or 7-hydroxy 4- methyl coumarin with various acylated aromatic amines in the presence of dry acetone as solvent. The acylated aromatic amines are prepared from various aromatic amines by treating with chloro acetyl chloride and acetic acid. Results: Ten coumarin derivatives (CPD-1- CPD-10) are synthesized characterized by NMR and mass spectral data and tested for in vitro anticoagulant activity by taking warfarin as standard. Conclusion: Coumarin derivatives are showing the moderate anticoagulant activity. Further can be tested for other biological activities.

Various substituted formazan derivatives received considerable importance during last decade as they are covered with wide variety of biological and pharmacological activities and have a wide range of therapeutic importance. Based on this a series of new formazan derivatives has been synthesized. Phenyl hydrazine was added drop wise to mixture of various substituted Aromatic aldehyde in dilute acetic acid forms various substituted Phenyl hydrazones. The solution of substituted Phenyl hydrazones in pyridine was added to diazonium salt solution such as hetero aryl amine (2-amino pyridine, 2-amino pyrazine) forms a novel various coloured formazan derivatives. The synthesized compounds were characterized by physical studies, like solubility, melting point, TLC and subjected to spectral studies like IR, 1H-NMR and mass spectroscopy. All the synthesized compounds were screened for In-vitro antioxidant activity was performed by the DPPH scavenging method by using Ascorbic acid as reference standard. Results suggested that electron withdrawing groups like nitro, chloro containing compounds shown potent antioxidant. Rest of the compounds showed mild to moderate activity.

A robust, simple precise and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Lamotrigine and Valproate in a combined dosage form. The stationary phase selected was C18 (150x4.6, 5µm) and the mobile phase comprising of Phosphate buffer (0.05M) pH 4.6: ACN (30:70%v/v), the flow rate was set at 1.0 ml/min. The analyte was measured in UV detector at 255 nm. The retention time of Lamotrigine and Valproate eluted were to be 2.551 and 4.879 min respectively. The linearity range was found to lie from 10µg/ml to 50 µg/ml of Lamotrigine and 20µg/ml to 100µg/ml of Valproate. The percentage assay of pharmaceutical formulation was obtained for Lamotrigine 100.7% and Valproate 101.4%. The proposed method was validated as per the ICH guideline and the method was linear, precise, accurate and specific.

Wound is defined as the damage of epidermic cells either by a cut or by any other damage. Wound healing responses to involving migration, proliferation, differentiation, and apoptosis of cell types. There are millions of plants in India to cure the diseases since ancient times. These plants were used because of their antioxidant property. 80% of world’s population depends upon herbal plants, according to WHO. The origin of Curcumalonga was found in southern part of Asia. Curcumalonga is also known as turmeric. Curcumalonga belongs to ginger family (Zingiberaceae). The medicinal value of curcumalonga is high. Curcumalonga also has the effect of invigorating the circulation of blood, relieving pain, clearing heat of heart and cooling blood, and curing jaundice. The presence of anti-inflammatory, anti-human immunodeficiency properties, anti-bacterial property, antioxidant effects and nematocidal activities made it as of high medicinal value. Turmeric powder consumption reduces the risk of various types of cancers. Curcumalonga helps the cells to grow faster.